Royal Rife's Laboratory Research on Bacillus "X" cancer virus
The BX was isolated from ten different cases of breast carcinoma by Dr. Royal Raymond Rife at the Rife Research Laboratory in SanDiego (Point Loma), California. It was carried through forty-four transplants on "K" media in all ten instances.
The technique used in the isolation of this organism is in brief as follows;
blocks of tissue, taken under the most sterile
conditions, were transferred into "K" media (previously examined for sterility). These were then placed under the direct
influence of an argon filled gas tube working under five thousand volts for twenty four hours, then were placed in water baths
with two inches of vacuum, and incubated at 37.5°C. At this time the delicate shine of growth is noticeable. From this point on
as many as desired transplants can be made without repeating the foregoing operations.
The BX is a filterable virus, which filters through the W Berkfeld filter.
It is a small ovoid granule, highly plastic, and visible only
with monochromatic light. The angle of refraction is 12 3/10°, and the color by chemical refraction is purple red. The length of
the organism is 1/15 u, and its breadth 1/20 u. It carries an attraction to the cathode pole. Its death rate in milliamperes is 175
DC. The X-ray and Infrared have no influence on the organism, but the Ultraviolet ray slows up its motility. The thermal death
point is 42°C for 24 hours, the filament voltage is 10, and the filament amperage is 86. The plate voltage is 928, and its
unmodulated electronic oscillatory rate is 11,780,000 cycles per second. The wavelength of super regeneration of Audion tube is 17 6/10 meters.
An inoculating serum was prepared by combining in a mixture, the transplants from the ten original growths with a (?) to 1 dilution of normal saline solution.
(The symbol (?) indicates that the text was cropped out in the photocopy.)
On Aug. 3, 1933 1/10 cc of the above serum was inoculated into the breasts
of two sets of white rats; one set consisting of
two pregnant females with one control, and the other of two young females and one control. The animals had been kept in
quarantine for a period of ninety days and were normal in every respect at time of inoculation. Seven days later the inoculated
rats developed lesions (superficial) in the thyroid region and on the shoulders. These lesions varied in size and severity on
succeeding days. The controls remained normal. On Aug. 21, the control of the pregnant female set gave birth to two young;
one died. The delivery of the inoculated pregnant females was still delayed, and the temperatures of all the inoculated animals
rose from 1 to 1 1/2° F. The lesions increased in area and density, and one in particular was decidedly elevated. On Aug. 22,
one of the infected rats presented 5 young, and the other until this day has remained barren, the swelling of the abdomen which
evidently was occupied by the young having gone down and returned to normal. In the offspring of the infected mother, two
developed the identical type of lesions on the surface of the thyroid region. One of these grew otherwise normally and in the
other the growth was stunted. The latter developed a severe growth on the upper portion of the right side of the jaw, which
consumed most of the normal tissue. The teeth were badly malformed, and grew very long, curving down and deep into the
throat. These were shortened by surgical operation. During this entire period the controls remained normal.
On Aug. 28, a set of male rats consisting of the same number was inoculated
as in the females. The same type of epidermal foci
developed, the control remaining normal. On Sept. 5, one of the males was posted and revealed no pathology. A lesion was
excised from the shoulder of the other inoculated male. (*) On Sept. 14, the Bacillus X was recovered and identified in the
media. The lesions on all the inoculated rats vary in size and density from day to day and in some cases clear up and break out
in other portions of the epidermis.
(*) Tissue placed in "K" media and run through the original method of technique.
It has been demonstrated by experiment that the BX exists in two cycles, which
may be classified as forms A and B. Form A
applies to BX in its ultra filterable cycle. In this stage the organisms theoretically exist in malignant tissue. Examination of the
fresh filtrate preparation of malignant tissue under 20,000X magnification, using any known system of illumination, fails to reveal
the presence of living bodies. However, after a special method of cultivation, involving the use of the argon ray and vacuum
conditions, the aforementioned filtrate in "K" medium contains a swarming myriad of the visible cycle, form B. The BX in this
form may be seen under 8-11,000X magnification, (using monochromatic illumination) as a highly plastic ovoid granule, purple
red in color.
Since experiments show that the Bacillus X in form A exists in malignant tissue,
it is theoretically possible to change its cycle to
form B by application of the argon ray and vacuum conditions. After the cycle change has been accomplished (in theory), the
application of the oscillative ray at a cycles per second vibration of 11,780,000 should completely destroy the BX in the
(note: the frequency listed here is the base frequency that undergoes modification
by the audio frequency that isn't listed here which is believed to be 2127 or